8.1 computer code Search Results


dsdna illumina-m13f bar-code  (Illumina Inc)
90
Illumina Inc dsdna illumina-m13f bar-code
A.) The bridging primers can be produced in house using standard PCR and purification. Individual, uniquely bar-coded bridging primers are amplified from the FREQ-Seq plasmid library set using two primers ABC1 ( AATGATACGGCGACCAC ) and ABC2 ( ACTGGCCGTCGTTTTAC ). The resulting bridging primers are stored as <t>dsDNA</t> at −20°C. B.) Construction of FREQ-Seq Illumina libraries is conducted in two stages. A first round of PCR amplification is used to generate an allele-specific, representative fragment pool with product inserts approximately 250 bp in size. Each product at this stage contains an <t>M13f</t> sequence at the sequencing end and an Illumina B adapter sequence at the non-sequencing end. These products are then treated with exonuclease I or Ampure XP beads to remove remaining PCR primers. In a second stage of PCR amplification, individual samples are barcoded and enriched using three primers; a small amount of a FREQ-Seq bridging primer and two Illumina enrichment primers. Initially, amplification proceeds via the sample-specific bridging primer that has the M13f sequence at its 3′ end. The resulting products can then be amplified to higher quantity with the generic, enrichment primers A and B. Following sample pooling and standard PCR product purification, the resulting products constitute a complete FREQ-Seq library.
Dsdna Illumina M13f Bar Code, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dsdna illumina-m13f bar-code/product/Illumina Inc
Average 90 stars, based on 1 article reviews
dsdna illumina-m13f bar-code - by Bioz Stars, 2026-04
90/100 stars
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ion xpress tm bar code adapters 81 96 kit  (Thermo Fisher)
86
Thermo Fisher ion xpress tm bar code adapters 81 96 kit
A.) The bridging primers can be produced in house using standard PCR and purification. Individual, uniquely bar-coded bridging primers are amplified from the FREQ-Seq plasmid library set using two primers ABC1 ( AATGATACGGCGACCAC ) and ABC2 ( ACTGGCCGTCGTTTTAC ). The resulting bridging primers are stored as <t>dsDNA</t> at −20°C. B.) Construction of FREQ-Seq Illumina libraries is conducted in two stages. A first round of PCR amplification is used to generate an allele-specific, representative fragment pool with product inserts approximately 250 bp in size. Each product at this stage contains an <t>M13f</t> sequence at the sequencing end and an Illumina B adapter sequence at the non-sequencing end. These products are then treated with exonuclease I or Ampure XP beads to remove remaining PCR primers. In a second stage of PCR amplification, individual samples are barcoded and enriched using three primers; a small amount of a FREQ-Seq bridging primer and two Illumina enrichment primers. Initially, amplification proceeds via the sample-specific bridging primer that has the M13f sequence at its 3′ end. The resulting products can then be amplified to higher quantity with the generic, enrichment primers A and B. Following sample pooling and standard PCR product purification, the resulting products constitute a complete FREQ-Seq library.
Ion Xpress Tm Bar Code Adapters 81 96 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ion xpress tm bar code adapters 81 96 kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
ion xpress tm bar code adapters 81 96 kit - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier
nvivo software version 9.2.81.0  (qsr international)
90
qsr international nvivo software version 9.2.81.0
A.) The bridging primers can be produced in house using standard PCR and purification. Individual, uniquely bar-coded bridging primers are amplified from the FREQ-Seq plasmid library set using two primers ABC1 ( AATGATACGGCGACCAC ) and ABC2 ( ACTGGCCGTCGTTTTAC ). The resulting bridging primers are stored as <t>dsDNA</t> at −20°C. B.) Construction of FREQ-Seq Illumina libraries is conducted in two stages. A first round of PCR amplification is used to generate an allele-specific, representative fragment pool with product inserts approximately 250 bp in size. Each product at this stage contains an <t>M13f</t> sequence at the sequencing end and an Illumina B adapter sequence at the non-sequencing end. These products are then treated with exonuclease I or Ampure XP beads to remove remaining PCR primers. In a second stage of PCR amplification, individual samples are barcoded and enriched using three primers; a small amount of a FREQ-Seq bridging primer and two Illumina enrichment primers. Initially, amplification proceeds via the sample-specific bridging primer that has the M13f sequence at its 3′ end. The resulting products can then be amplified to higher quantity with the generic, enrichment primers A and B. Following sample pooling and standard PCR product purification, the resulting products constitute a complete FREQ-Seq library.
Nvivo Software Version 9.2.81.0, supplied by qsr international, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nvivo software version 9.2.81.0/product/qsr international
Average 90 stars, based on 1 article reviews
nvivo software version 9.2.81.0 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


A.) The bridging primers can be produced in house using standard PCR and purification. Individual, uniquely bar-coded bridging primers are amplified from the FREQ-Seq plasmid library set using two primers ABC1 ( AATGATACGGCGACCAC ) and ABC2 ( ACTGGCCGTCGTTTTAC ). The resulting bridging primers are stored as dsDNA at −20°C. B.) Construction of FREQ-Seq Illumina libraries is conducted in two stages. A first round of PCR amplification is used to generate an allele-specific, representative fragment pool with product inserts approximately 250 bp in size. Each product at this stage contains an M13f sequence at the sequencing end and an Illumina B adapter sequence at the non-sequencing end. These products are then treated with exonuclease I or Ampure XP beads to remove remaining PCR primers. In a second stage of PCR amplification, individual samples are barcoded and enriched using three primers; a small amount of a FREQ-Seq bridging primer and two Illumina enrichment primers. Initially, amplification proceeds via the sample-specific bridging primer that has the M13f sequence at its 3′ end. The resulting products can then be amplified to higher quantity with the generic, enrichment primers A and B. Following sample pooling and standard PCR product purification, the resulting products constitute a complete FREQ-Seq library.

Journal: PLoS ONE

Article Title: FREQ-Seq: A Rapid, Cost-Effective, Sequencing-Based Method to Determine Allele Frequencies Directly from Mixed Populations

doi: 10.1371/journal.pone.0047959

Figure Lengend Snippet: A.) The bridging primers can be produced in house using standard PCR and purification. Individual, uniquely bar-coded bridging primers are amplified from the FREQ-Seq plasmid library set using two primers ABC1 ( AATGATACGGCGACCAC ) and ABC2 ( ACTGGCCGTCGTTTTAC ). The resulting bridging primers are stored as dsDNA at −20°C. B.) Construction of FREQ-Seq Illumina libraries is conducted in two stages. A first round of PCR amplification is used to generate an allele-specific, representative fragment pool with product inserts approximately 250 bp in size. Each product at this stage contains an M13f sequence at the sequencing end and an Illumina B adapter sequence at the non-sequencing end. These products are then treated with exonuclease I or Ampure XP beads to remove remaining PCR primers. In a second stage of PCR amplification, individual samples are barcoded and enriched using three primers; a small amount of a FREQ-Seq bridging primer and two Illumina enrichment primers. Initially, amplification proceeds via the sample-specific bridging primer that has the M13f sequence at its 3′ end. The resulting products can then be amplified to higher quantity with the generic, enrichment primers A and B. Following sample pooling and standard PCR product purification, the resulting products constitute a complete FREQ-Seq library.

Article Snippet: To create the plasmid-borne bar-code library, 100 ng of the linear pUC19 PCR product as well as an equimolar amount of the 81 bp dsDNA Illumina-M13f bar-code were mixed and assembled into circular plasmid DNA using the one-step method developed by Gibson and co-workers .

Techniques: Produced, Purification, Amplification, Plasmid Preparation, Sequencing